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Cell Count & Viability Helper

Process manual hemocytometer slide grid counts, evaluate live/dead viability fractions, and calculate cell dilution volumes for plate seeding experiments.

Source: Trypan Blue Membrane Exclusion StandardsSource: Standard Hemocytometer Counting Grid (0.1 mm depth)

Slide Grid Entry

4 Squares
Square 1

Live Cells

Dead Cells

Square 2

Live Cells

Dead Cells

Square 3

Live Cells

Dead Cells

Square 4

Live Cells

Dead Cells

2 for 1:1 Staining

Default 10^4 (0.1 mm)

mL

Calculation Output

Live Cell Concentration9.750e+5cells / mL
Total Cell Concentration1.065e+6cells / mL
Viability Rate91.5%
Total Viable Cells4.875e+6in 5 mL volume
Avg Live / Square48.8cells

Mathematical Formula & Assumptions

Cells / mL = Average cells per square × Dilution factor × 10^4
Key Assumptions & Rules:
  • Chamber volume factor is default 10^4, assuming a standard depth of 0.1 mm and square grid dimensions of 1 mm x 1 mm (0.1 mm³ per square).
  • Viability is computed using trypan blue exclusions: viable cells divided by total counted cells.
  • Optimal loading limits per square: 20 to 100 cells. Counts outside this range trigger density advisory messages.
Research Use OnlyThis calculator operates entirely in your browser. Verify calculations, reagent formulations, cell counts, and dilutions against your lab standard operating procedures (SOPs), supplier datasheets, and reference literature. Do not base medical diagnoses, clinical actions, or patient care decisions on this toolkit.

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