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Buffer & Media Recipe Maker

Prepare custom volume mixtures for PBS buffers, sterile media solutions, and cell activation cocktails. Scale amounts instantly and check chemical safety warnings.

Source: Cold Spring Harbor Protocols (CSH)Source: Standard Laboratory Staining Rules

Select & Scale Buffer

1× Phosphate-Buffered Saline (PBS)

Scaled to 1 L

Ingredients List

Ingredient NameRequired AmountNotes
Sodium Chloride (NaCl)8 g-
Potassium Chloride (KCl)0.2 g-
Disodium Phosphate (Na2HPO4)1.44 g-
Monopotassium Phosphate (KH2PO4)0.24 g-
Deionized Water (dH2O)1 LAdd up to final volume

Directions / Preparation Steps

  1. Dissolve dry ingredients in ~800 mL deionized water.
  2. Adjust pH to 7.4 using HCl or NaOH.
  3. Add deionized water to reach a final volume of 1.0 L.
  4. Autoclave on liquid cycle (20 mins at 121°C) or filter sterilize (0.22 µm) for cell culture use.
Storage & PreservationStore at Room Temperature (15-25°C) or 4°C. Stable for several months.
Research Use OnlyThis calculator operates entirely in your browser. Verify calculations, reagent formulations, cell counts, and dilutions against your lab standard operating procedures (SOPs), supplier datasheets, and reference literature. Do not base medical diagnoses, clinical actions, or patient care decisions on this toolkit.

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