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Antibody Dilution & Molarity Calculator

Convert antibody mass concentrations to molarity, calculate C1V1 bench dilutions, and prepare multi-sample staining master mixes.

Source: Harlow & Lane Antibodies Laboratory ManualSource: Molecular Weight Assumptions

Input Parameters

kDa
Standard MW Presets

Calculation Output

Molarity (nM)6.67 nM
Molarity (µM)0.00667 µM
Molecules per mL4.0148e+12molecules / mL

Mathematical Formula & Assumptions

Molarity (M) = Concentration (g/L) / Molecular Weight (g/mol)
Key Assumptions & Rules:
  • Intact IgG molecular weight is assumed to be ~150 kDa.
  • IgG Fab fragment molecular weight is assumed to be ~50 kDa.
  • Concentration conversions: 1 µg/mL = 1 mg/L.
  • Avogadro's number represents 6.022 × 10^23 molecules per mole.
Research Use OnlyThis calculator operates entirely in your browser. Verify calculations, reagent formulations, cell counts, and dilutions against your lab standard operating procedures (SOPs), supplier datasheets, and reference literature. Do not base medical diagnoses, clinical actions, or patient care decisions on this toolkit.

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